UV intensity detector

The working principle of the instrument is based on the law of light absorption. The light emitted from the light source passes through a slit, a filter, and a sample cell before reaching the photoelectric amplifier tube. The change in light intensity caused by different sample concentrations is converted into a change in photocurrent. This photocurrent is then input into a logarithmic converter through an amplifier, which converts the transmittance T into an output of A, i.e. A=1g -=ε CL, where ε is the molar extinction system of the sample to be tested, C is the sample concentration, measured in grams per liter, and L is the optical path length, measured in centimeters. According to the above formula, it can be determined that A has been measured, and the sample concentration C is known. If the amplifier is directly input into the recorder, the graph of the change in sample transmittance T is drawn. If the logarithmic converter is input into the recorder, the graph of the change in sample optical density A is drawn.

UV intensity detectorUsage

1. Firstly, connect the circuits of the host and recorder, and then plug the power plug of the host into a 220V power outlet.

2. The wavelength is set to 280nm before leaving the factory. If other wavelengths are required, simply turn the wavelength conversion knob on the right side of the instrument to the desired experimental setting.

3. Press the power switch of the host and the indicator light will turn on. If conducting a transparency T test, turn the knob (i.e. sensitivity) to the T position. Adjust the light knob, and the LED digital display board will also display the number at the same time.

4. If conducting an optical density A test, turn the range knob (i.e. sensitivity) to the T position required for the experiment. Adjust the light knob so that the LED digital panel displays around 98. Preheat the instrument for about 1.5 hours, and once the baseline is stable, calibrate the instrument.

5. Connect the plastic tube at the bottom of the chromatography column to the injection plastic tube of the host, and connect the output plastic tube to a partial collector or test tube. At this time, there is eluent passing through the sample pool.

6. Adjust the light intensity knob and place the range knob in T position to display the LED number as 100. Turn T to A position to read around 0 on the LED panel. If conducting a transmittance T test, you can add a sample to the chromatography column for separation and analysis of the sample.

7. If conducting an optical density A test, turn the range knob (i.e. sensitivity) to the desired optical density A, adjust the light quantity knob to make the LED panel 0. At this point, the recorder returns to near zero (note: near zero is sufficient, which can be determined in the experiment).

8. If the peak value of the graph is too small, turn the range knob (i.e. sensitivity) to the desired position.

9. During the testing process, the 'light intensity' remains unchanged. Test completed, and clean the sample tank and pipeline with distilled water.