Product Introduction

The Trypsin EDTA Solution produced by Soleibao contains 0.25% trypsin and 0.02% EDTA (0.53mM), dissolved in a calcium free magnesium balanced salt solution. After filtering out the bacteria, it can be directly used for digestion of cultured cells and tissues. This product has the characteristics of convenience, speed, stability, safety, and good cell state.

Product Description:

In vitro culture and primary cell culture of tissue cells, tissue cell dispersion (preparing tissue blocks into single cell suspensions), as well as in passage cell culture, tissue cell digestion solution is used for the digestion and dispersion of adherent growth cells. The commonly used digestive juices are trypsin, EDTA, etc. Their main function is to hydrolyze proteins between cells (such as extracellular matrix), disperse tissues or adherent cells into individual cells, and make cell suspensions for further experiments.

Storage: stored at -20 ℃, valid for 12 months.

usage method:

1. Digestion of adherent cells:

a) Suck off the culture medium and wash the cells once with sterile PBS, Hanks' solution, or serum-free culture medium to remove residual serum.

b) Add a small amount of trypsin digestion solution, cover the cells, and leave at room temperature for 1-2 minutes. Different cells have different digestion times, and for cells that adhere firmly to the wall, the digestion time can be appropriately extended.

c) Under the microscope, the cells visibly contracted, and upon visual inspection of the bottom of the culture vessel, significant changes in the morphology of the cells were observed; Or use a gun to blow the cells and find that the cells can just be blown down. At this point, remove the digestive fluid. Add cell culture medium containing serum, blow off the cells, and they can be directly used for subsequent experiments.

d) If insufficient digestion is found, trypsin digestion solution can be added for re digestion.

e) If it is found that the digestion time of the cells is too long and some of the cells have already fallen off from the bottom of the culture vessel before being blown, use trypsin cell culture medium to blow down all the cells directly. Centrifuge 1000-2000g for 1 minute, precipitate the cells, try to remove trypsin cell digestion solution as much as possible, add complete culture medium containing serum to resuspend the cells, and they can be used for subsequent experiments.

2. Organizational digestion:

The time required for digestion varies greatly among different tissues, and it is usually advisable to fully disperse the tissues after digestion.

matters needing attention:

Due to differences in tissue or cell properties, experimenters should determine the optimal digestion time based on specific circumstances; The digestion time of cells should not be too long, otherwise it will affect the cell adhesion and growth status;

2. This product does not contain antibacterial agents. During use, special attention should be paid to aseptic operation to avoid contamination of digestive fluids by microorganisms;

3. It is not advisable to store at 4 ℃ for a long time, and repeated freezing and thawing should be avoided. When used in small quantities, it is recommended to pack and store separately;

For your safety and health, please wear lab clothes and disposable gloves when operating.