SUPERCOSIL LC-18 HPLC Column Liquid Chromatography Column:

Product features:

SUPERCOSIL LC-18 HPLC Column:

Having retention and providing good peak shapes for a wide range of compoundsUniversal hydrophobic alkyl phase.

SUPERCOSIL LC-18 HPLC Column Ordering Specification:

Related information:

Liquid chromatography column is a component used in liquid chromatography analysis instruments;

Before choosing a chromatographic column, it is important to understand your own sample and impurities, including their type, structure, polarity, acidity, molecular weight, and so on,

1. The sample is polar and weakly acidic; C18 can be selected for detection under 100% acidic aqueous solution conditions, that is, a chromatographic column that can withstand 100% pure water and retain polar compounds well should be chosen

If the polarity of the sample is too strong or the acidity is too strong; You can choose CN, NH2， Or silica gel columns, HILIC (hydrophilic chromatography), there are also C18+strong anion pair reagents or strong anion exchange chromatography columns used (the disadvantage is that the ion pair reagent has a long equilibrium time, the pH of the mobile phase requires precision, otherwise it is difficult to repeat the experiment, and the ion pair reagent is difficult to wash off. Basically, chromatography columns that use ion pairs cannot be used for other experiments)

3. If the sample is alkaline; You can choose high-purity silica gel columns (high-purity silica gel lacks metal impurities and has silica gel end caps) or some modified C18 columns (such as polar embedding technology or alkali deactivation technology), both of which can reduce the tailing of alkaline compounds. Generally, neutral or slightly alkaline conditions are chosen to increase the retention of alkaline samples

If the polarity of the alkaline compound is too strong or the alkalinity is too strong; You can choose a wide pH C18 chromatographic column for high pH detection (the advantage is that the method development is simple, but the disadvantage is that there are relatively few chromatographic column brands and high prices currently available for this technology), or use a HILIC chromatographic column (a silica gel column used under reverse phase conditions, which is also a classic method for detecting alkaline samples). You can also choose a strong ion exchange column (the disadvantage is that it cannot be used to analyze other samples, and the pH of the mobile phase requires precision, otherwise it is difficult to repeat the experiment). C18+strong anion pair reagents or strong anion exchange chromatographic columns are also used.