DH35-250 is an ultrasonic equipment used in laboratories and small batch liquid processing, fully utilizing the advantages of digital integrated circuits. Compared with traditional ultrasonic tissue processors, the instrument is more stable and reliable, with a smaller volume, saving laboratory space, and equipped withElectric lifting workbenchThe lighting function makes experimental operations safer and more convenient.

It is mainly suitable for processing techniques such as crushing&crushing, dispersion&depolymerization, extraction&extraction, emulsification, homogenization&mixing, sonochemistry, decomposition, etc.

◆ The soundproof box is equipped with an electric lifting workbench for safe and convenient experimental operations.

◆ Equipped with lighting device inside the soundproof box - convenient for observing experimental effects.

The ultrasonic generator and soundproof box are integrated to save laboratory space.

The host adopts a digital intelligent ultrasonic generator (core technology reflected).

Ⅰ: Automatic frequency search range up to ± 500Hz, users can verify on their own to prevent fraud - core technology reflected

II: Automatic frequency tracking range up to ± 500Hz, users can verify on their own to prevent fraud - core technology reflected

III: Frequency tracking accuracy reaches 1Hz and real-time display - core technology embodiment

Improve the stability of instrument operationWhen the ultrasound tissue processor is turned on and starts ultrasound, the host, i.e. the ultrasound generator, will automatically search for the natural frequency of the transducer+amplitude rod (or tool probe). The wear and tear of the amplitude rod, the aging of the transducer, and the replacement of the amplitude rod (which is a consumable) can cause significant changes in the natural frequency value. The simulated ultrasonic generator also has a certain frequency automatic search ability at the moment of starting the ultrasonic wave, but the search ability is poor (about ± 150Hz). When the natural frequency value is large, the simulated circuit ultrasonic generator can no longer search for the same frequency as the transducer+amplitude rod. At this time, the ultrasonic operation is turned on, which is called a detuned state. If the instrument works in a detuned state, the components and transducers inside the ultrasonic generator generate a large amount of heat, and the stability of the whole machine's operation becomes worse, greatly affecting the service life of the instrument and the repair rate is high. At present, although some manufacturers use manual switches to adjust inductance to adjust frequency detuning, it is undoubtedly a troublesome task for users because 1. The energy of ultrasonic cell disruptors is focused, with a narrow bandwidth, making it difficult to adjust; 2. The detuning state is a gradual process, and you cannot know when it is most appropriate to adjust the inductance. At the moment of startup, the digital intelligent ultrasonic generator developed by our company has an automatic frequency search range of ± 500Hz, which is more than three times the frequency search ability of analog circuit ultrasonic generators. This greatly improves the instrument's adaptability to frequency changes, especially when the ultrasonic generator exceeds its automatic frequency search range, the instrument does not start working, effectively protecting the instrument, so the maintenance rate is extremely low.

Improve the reliability of instrument operationAfter the instrument enters normal operation, the ultrasonic generator tracks the frequency of the transducer and amplitude rod in real time and provides a frequency that is consistent with it. This frequency is also known as the resonant frequency, and the instrument works most efficiently and stably in this state. After entering work, the frequency of the transducer+amplitude rod changes in real time, mainly affected by changes in the temperature of the processed material, wear of the amplitude rod, and heating of the transducer. This requires the ultrasonic generator to have the ability to track the frequency of the transducer+amplitude rod in real time over a large range. At present, the vast majority of ultrasonic cell disruptors on the market are equipped with analog circuit ultrasonic generators. Although they also have a certain frequency automatic tracking function for (transducer+amplitude rod), the tracking range value is relatively small (about ± 150Hz). However, the digital circuit intelligent ultrasonic generator used by our company can achieve a frequency tracking range of ± 500Hz, greatly improving the stability and reliability of the instrument during operation! When the simulated ultrasonic generator exceeds its frequency tracking capability range during ultrasonic operation, the instrument can still operate ultrasonically (it should be stopped immediately), because the (transducer+amplitude rod) is no longer in the optimal working state, and the ultrasonic efficiency gradually decreases, causing the ultrasonic generator and transducer to generate more heat, and the overall operation of the machine becomes increasingly unstable, resulting in a higher failure rate. The frequency automatic tracking capability of the digital intelligent ultrasonic generator launched by our company is more than three times higher than that of the analog ultrasonic generator. Moreover, when the all digital intelligent ultrasonic generator exceeds its frequency tracking range, the instrument can automatically shut down, effectively protecting the instrument, so the maintenance rate is extremely low.

Improve material handling efficiencyWhether the ultrasonic generator accurately tracks the frequency of the transducer and amplitude rod will affect the ultrasonic output intensity, thereby affecting the effectiveness of material processing. The analog ultrasonic generator has inaccurate frequency tracking for the transducer and amplitude rod, while the digital intelligent ultrasonic generator has very accurate frequency tracking for the transducer and amplitude rod, with an accuracy of up to 1Hz and better material processing effect.

Organizational processing methods:

1、 Mechanical processing method: refers to the use of crushers, grinders, or homogenizers to break down cells.

1. High speed organization crushing: Mix the material into a thin paste like liquid, place it in a cylinder with about 1/3 volume, cover the cylinder tightly, turn the speed controller to the slowest position first, turn on the switch, and gradually accelerate to the desired speed. This method is applicable to animal visceral tissues, plant fleshy seeds, etc.

2. Glass homogenizer homogenization: First, place the shredded tissue in a tube, then insert it into a grinding rod and grind it back and forth. Move it up and down to crush the cells. This method has a higher degree of cell fragmentation than high-speed tissue crushers and is suitable for small quantities and animal organ tissues.

2、 Physical fragmentation method: refers to the use of temperature, pressure, or ultrasonic waves to break cells apart.

1: Treat cell suspension with ultrasound of a certain power to cause rapid shaking and rupture of cells (breaking cell walls and organelles with the vibration force of ultrasound).

Mechanism: It may be related to the cavitation phenomenon caused by the generation, growth, and fragmentation of bubbles when strong sound waves act on the solution. The shock wave and shear force caused by cavitation cause cell lysis.

The efficiency of ultrasonic fragmentation depends on factors such as sound frequency, acoustic energy, processing time, cell concentration, and cell type. (Pay attention to cooling during use to prevent overheating).

2. High pressure fragmentation: The cell suspension is sprayed from the annular gap of the high-pressure chamber onto the stationary impact ring, and is forced to change direction and flow out through the outlet pipe. During this process, cells undergo high-speed shear collisions and changes from high pressure to normal pressure, thereby breaking down and releasing their contents.

This is an ideal method for gently and thoroughly breaking down cells.

3. Repeated freeze-thaw method: Freeze the cells below -20 degrees Celsius, thaw at room temperature, repeat several times. Due to the formation of ice particles inside the cells and the increase in salt concentration in the remaining cell fluid, swelling occurs, causing the cell structure to break down.

3、 Chemical fragmentation method: refers to the use of organic solvents or surfactants such as formaldehyde and acetone to act on the cell membrane, causing the structure of the cell membrane to be damaged or the permeability to change.

Some animal cells, such as tumor cells, can be disrupted by cell membranes using sodium dodecyl sulfate (SDS), sodium deoxycholate, and other agents. The concentration is generally 1mg/ml.

4、 Enzymatic fragmentation method: Select appropriate enzymes to destroy the cell wall, and then break down the protoplasts in a hypotonic solution.

Bacterial cell walls are thicker and can be treated with lysozyme for better results.

Standard formula for cracking solution: 50mM Tris HCl (pH 8.5-9.0), 2mM EDTA, 100mM NaCl, 0.5% Triton X-100, 1mg/ml lysozyme. Lysozyme works better within this pH range.

Comprehensive description: Regardless of which method is used to crush tissue cells, it will release intracellular proteins or nucleic acid hydrolytic enzymes into the solution, causing the biodegradation of large molecules and reducing the mass of natural substances. Adding diisopropylfluorophosphate (DFP) can inhibit or slow down autolysis; Adding iodoacetic acid can inhibit the activity of proteolytic enzymes that require hydrophobic groups in their active centers. Adding phenylsulfonyl fluoride (PMSF) can also remove the activity of proteolytic enzymes, but not all of them, and should be added several times while crushing; In addition, pH, temperature, or ionic strength can be selected to make these conditions suitable for the extraction of the target substance.

Precautions before use:

1. Select an appropriate probe based on the volume of the sample being processed, and wipe the probe with an alcohol cotton ball before and after use. To replace the probe, use a specialized wrench;

When the AMPLITUDE knob is turned on, the probe should not be exposed to air, and in special circumstances (test probe), it should not exceed 10 seconds;

3. Prepare an ice box before use, and the sample must be placed in an ice bath during processing. The sample concentration should not be too high.