Leica TCS SP8;

Nikon A1/C2;

Olympus FV1000/FV1200/FV3000/FVMPE-RS;

Zeiss LSM 710/LSM 780;

These confocal microscopes can be easily upgraded to fluorescence lifetime imaging FLIM/FFS functionality.

Adopting photon counting detection and optional TCSPC time-domain technology for data acquisition.

After upgrading, you can obtain:

1. Time resolution of 1ps

2. Data collection capability of 100ps-100ms;

3. 8-channel detection capability;

4. 65MHz/channel

5. NDD two-photon detector;

The FFS function includes:

Fluorescence Correlation Spectroscopy (auto- and cross-correlation FCS)

Photon Counting Histogram (PCH)

FFS measurements at target XYZ locations in an image

FLCS, Fluorescence Lifetime Correlation Spectroscopy

Number & Brightness (N&B)

Figure 1. Schematics of the upgrade package for the Leica TCS SP8 Frequency-Domain Confocal Microscope. The part to the right includes the instrument components (PC, control electronics, scanner and laser launcher). The left part of the schematics includes the components provided by ISS with the upgrade package.