The method for detecting ethanol content in blood is to use tert butanol as an internal standard, and then use headspace gas chromatography flame ionization detector to detect the sample. Compared with the ethanol standard operated in parallel, the internal standard method is used to quantify the peak area ratio of ethanol to the internal standard. The target peak on the chromatogram is separated from the internal standard peak, and the standard working curve has a good linear relationship within the range of 0.01-10.0 g/L. The headspace gas chromatography method is rapid, sensitive, and accurate for determining ethanol content in blood. This method can be used for practical detection and analysis.
The concentration of ethanol in the blood is an important indicator for determining a person's level of alertness.
Instrument configuration:
product name |
Specification and model |
unit |
quantity |
gas chromatograph |
GC-9890E FID capillary injection |
cover |
1 |
Headspace sampler |
HS-16A |
cover |
1 |
Workstation software and interfaces |
SD-2020 |
cover |
1 |
capillary column |
PEM-20M 0.53um*30m |
root |
1 |
hydrogen generator |
SDH-1 headspace sampler |
tower |
1 |
air pump |
SDA-1 oil-free type |
tower |
1 |
Standard sample |
|
|
|
volumetric flask |
|
|
|
Pipette |
|
|
|
Headspace auxiliary accessories |
1 sealing pliers, 50 imported headspace bottles, 300 aluminum caps, and 50 injection pads |
cover |
1 |