Detection of fructose, glucose, maltose, and sucrose content in honey

Brand: SCH (scallop shell) Origin: Germany Domestic Agent: Xuan Sheng Ke Yi

Honey is one of the classic health products with a long history. Pure and high-quality honey has high nutritional value and efficacy, so the selling price of good honey is also relatively high. This has also stimulated some illegal vendors to mix and falsify honey in order to make huge profits, seriously harming the interests of consumers. Therefore, the country introduced mandatory national standards for honey in 2006. The new standards mainly judge the quality of honey based on three mandatory indicators, namely moisture, total fructose and glucose, and sucrose content. If a company illegally adds syrup, white sugar and other ingredients to honey, it will inevitably result in unqualified sugar, soDetection of sugar content in honey by liquid chromatographyIt is of great significance.

2015 Pharmacopoeia SpecificationDetection of sugar content in honey by liquid chromatographyThe 2015 edition of the pharmacopoeia requires revision of standards, including the detection of fructose, glucose, maltose, and sucrose content in honey as a medicinal excipient and edible food. [Content determination] Determine according to the high-performance liquid chromatography method (Appendix VI D).The chromatographic conditions and system suitability test were conducted using a Prevail Carb ES Column-W 250x4.6mm 5um chromatographic column with acetonitrile water (75:25) as the mobile phase; Differential refractive index detector (Shanghai Xuansheng recommends German scallop shell SCH RI 2000). The number of theoretical plates calculated based on fructose peak should not be less than 2000.

The preparation of the standard curve involves accurately weighing 1.0g of fructose reference substance and 0.8g of glucose reference substance, placing them in the same conical flask with a stopper. Then, 20ml of 40% acetonitrile is precisely added, dissolved, and shaken well to prepare the reserve solutions for fructose and glucose reference substances. Accurately weigh 0.2g of sucrose reference substance and 0.2g of maltose reference substance, and place them in the same conical flask with a stopper. Precisely add 10ml of 40% acetonitrile, dissolve, shake well, and use as reserve solutions for sucrose and maltose reference substances. Accurately measure the reserve solutions of fructose and glucose reference standards, as well as sucrose and maltose reference standards, and add 40% acetonitrile to prepare mixed reference solutions of different concentrations of fructose, glucose, sucrose, and maltose. Accurately extract 15 μ l of each mixed reference solution and inject it into the liquid chromatograph for separate measurements. Draw a standard curve with the concentration of the reference substance as the horizontal axis and the peak area value as the vertical axis, and calculate the regression equation. Preparation of test solution: Take about 1g of this product, weigh it accurately, place it in a conical flask with a stopper, add 20ml of 40% acetonitrile accurately, dissolve, shake well, filter, and take the filtrate to obtain. Measurement method: Accurately measure 15 μ l of the test solution, inject it into the liquid chromatograph, measure it, and calculate the content according to the standard curve method. The total amount of fructose (C6H12O6) and glucose (C6H12O6) in this product shall not be less than 60.0%, and the ratio of fructose to glucose content shall not be less than 1.0

SCH2000Characteristics of differential refractive index detector instrument

1. Advanced circuit and mechanical design enable SCH2000 to have * sensitivity, ultra-low noise, high baseline stability, and low maintenance. The instrument balance time is short, only about 30 minutes.

2. The fully digital circuit design makes SCH2000 not only a detector, but also an analog-to-digital conversion interface for other detectors, which can serve as the main system of the entire LC system;

SCH2000 has a built-in full graphic display screen to display detailed information, an internal buffer for chromatographic data memory, and a built-in digital interface;

SCH2000 can also be optionally equipped with a data receiving software package, which can be used on Windows 3. x, Used under Windows 95 or Windows NT/XP.

5. Upgraded components for solvent recyclers can be provided.

SCH2000Performance parameters of differential refractive index detector

Detection method: Refraction

Refractive index range: 1.00-1.75

Flow range: 0.2-3.0ml/min

Linear range: 80 × 10-5RIU

Noise level: 5 × 10-10RIU

Automatic zero range: 40 × 10-5RIU

Automatic zero point resolution: 5 × 10-10RIU

Integral meter output: ± 1V

Recorder output: ± 10V

Digital interface: A -5Vdc to 5VDC analog signal input

Digital input: an analog signal input ranging from -5Vdc to 5VDC

Output: 5VDC, TTL or 12VDC intensity, marker, temperature, solvent cycler

Input: 5VDC, TTL cleaning, polarity, automatic zeroing, marker, temperature, solvent cycler

Temperature setting: Rt， Fixed at 35 ℃ or 55 ℃ (microprocessor controller)

Flow pool volume: 9ul

Flow pool pressure: maximum 6kg/cm2