Product Introduction

DMEM is one of the commonly used culture media in cell culture today. DMEM medium is developed based on MEM medium, and compared with MEM medium, DMEM medium increases the amount of various components in MEM medium. The following mainly introduces the method of preparing DMEM by oneself.

1. Prepare fresh triple distilled water or Millipore ultrapure water.

Weigh the required amount of dry powder culture medium and add it to about half of the final volume of distilled water; If preparing a packaged culture medium, after pouring the entire packaging of dry powder into three distilled water, the inner surface of the packaging bag needs to be washed twice with water and poured into the culture medium to ensure that all dry powder is dissolved into the culture medium. Magnetic stirring or manual stirring to completely dissolve it.

Add the required amount of sodium bicarbonate according to the requirements on the packaging bag; Add HEPES (5-20mmol/L), glutamine, and other special substances as needed for the experiment.

Add water to the final volume.

Adjust the pH with 1 mol/L hydrochloric acid and 1 mol/L sodium hydroxide if necessary.

6. Filter and sterilize using a sterile 0.22um filter membrane, pack in sterile serum bottles, and store in a refrigerator at 4 ℃.

Add 100U/mL penicillin and 100U/mL streptomycin to the prepared culture medium before use, and add serum (5% -20%) as needed. DMEM culture medium is widely used. Based on the rich content of amino acids and microorganisms in DMEM medium, as well as the presence of pyruvic acid and trace Fe ions in glycolysis, it is most commonly used in cell culture, vaccine production, and other fields.