1、 Instrument usage

The African swine fever PCR detector (real-time fluorescence quantitative PCR instrument) is used to run virus detection experiments and analyze experimental data; The instrument can be operated in the laboratory or used for field scientific experiments. With corresponding reagents, it can quickly and accurately qualitatively detect target nucleic acids in analytes or other analytes taken from the sample to be tested.

The instrument is equipped with African swine fever virus nucleic acid rapid detection kit (fluorescence RPA method) and African swine fever virus nucleic acid detection kit (real-time fluorescence PCR method), which can meet the on-site rapid detection needs of African swine fever virus nucleic acid. Quantitative and rapid diagnosis of livestock diseases such as African swine fever, avian influenza, swine fever, porcine blue ear, pseudorabies, etc., widely used in breeding farms, slaughterhouses, food processing plants, meat product deep processing enterprises, Ministry of Agriculture and Rural Affairs, animal husbandry bureaus, inspection and quarantine units.

Experimenters need to undergo specialized training in laboratory techniques, instrument and software operations, and possess proficient operational skills.

2、 Instrument features

1. Small size, light weight, and easy to carry. Easily meet the needs of outdoor experiments.

2. Built in 7-inch high-definition capacitive screen PDA, touch screen operation, simple and fast.

3. Marlow high-quality Peltier refrigeration chips, combined with Germany's high-end PT1000 temperature sensor and temperature control mode with electric resistance heating compensation edge, have a maximum heating rate of 6 ℃ and a maximum cooling rate of 5 ℃, greatly reducing experimental time.

4. The whole board adopts a 3-second fast lighting mode to ensure the consistency of the hole positions in the experimental results.

5. Simple and intuitive software guidance, making it easy to start detection experiments.

3、 Application fields of African swine fever PCR detector

□ Basic scientific research

□ Pathogen detection

□ Meat products adulterated

□ Transgenic testing

□ Food safety testing

□ Drug development and rational drug use

□ Gene expression

□ Water monitoring

4、 Technical parameters

Sample capacity: 16x0.2ml, supports 8-tube connection

Applicable consumables: Common transparent PCR consumables, 8x0.2ml tube, 0.2ml single tube

Reaction system: 5-120ul reaction mode system

Heating/cooling module: imported semiconductor thermoelectric module

Temperature control range: 4 ° C-99 ℃

The average rate of temperature rise and fall is ≥ 2 ° C/second

Temperature control accuracy: ≤ ±0.1°C

Temperature uniformity: ≤ ± 0.2°C

Number of temperature control areas: Multiple points (2 points)

Number of gradients: 0

Gradient temperature range: none

Number of gradient holes: none

Excitation light source: maintenance free LED

Excitation light wavelength range: 400-700nm

Testing component: Imported photoelectric detector

Number of detection channels: standard configuration with 1 channel (FAM), high-end configuration with FAM and VIC

Applicable dyes and probes: FAM/SYBR Green I, VIC/HEX/CY3, ROX/Texas Red (optional), Cy5, TAMARA (optional)

Software function: Fluorescence quantitative PCR system software;

Real time amplification reaction curve function;

Real time response curve display of specific specimens;

Data analysis function;

Automatic judgment function for yin-yang results;

Graphic display function.

Noise:< 45 dB

Screen size: 7 inches (HD)

Touch screen: capacitive type

External USB: Supports data import and export

Hot cover: automatic pressure regulation

Exterior dimensions: 290 (W) * 308 (L) * 130 (H)

Box size: 75 cm in length, 38 cm in width, and 19 cm in height

Net weight: approximately 3Kg

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